多指标正交试验优化菊花提取工艺(3)
Ministry of Education， Nanjing University of Chinese Medicine， Nanjing 210029， China；
2. Nanjing Haichang Chinese Medicine Group Co.， Ltd.， Nanjing 210061， China； 3. Key Laboratory of Processing
Standardization of Traditional Chinese Medicine State Administration of Traditional Chinese Medicine， Nanjing 210046， China)
[Abstract] Objective： To explore quantitative assessment indicators of Chrysanthemi Flos， and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design. Method： The concentration of ethanol， amount of ethanol， extraction time and extraction frequency were selected as factors in the L9(34) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators. Result： The optimum extraction process was selected as follows： using ultrasonic extraction method， adding 30-fold ethanol with 80% concentration， extracting for 2 times for extraction， 40 min for each time. Conclusion： The optimized extraction process is reliable， with controllable assessment indicators， which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.
[Key words] Chrysanthemi Flos； orthogonal experiment； fingerprint； multi-index； extraction process
doi：10.4268/cjcmm20130609
[责任编辑 曹阳阳], http://www.100md.com(张星海 李嬛 秦昆明 刘晓 汪斌 蔡宝昌)
2. Nanjing Haichang Chinese Medicine Group Co.， Ltd.， Nanjing 210061， China； 3. Key Laboratory of Processing
Standardization of Traditional Chinese Medicine State Administration of Traditional Chinese Medicine， Nanjing 210046， China)
[Abstract] Objective： To explore quantitative assessment indicators of Chrysanthemi Flos， and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design. Method： The concentration of ethanol， amount of ethanol， extraction time and extraction frequency were selected as factors in the L9(34) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators. Result： The optimum extraction process was selected as follows： using ultrasonic extraction method， adding 30-fold ethanol with 80% concentration， extracting for 2 times for extraction， 40 min for each time. Conclusion： The optimized extraction process is reliable， with controllable assessment indicators， which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.
[Key words] Chrysanthemi Flos； orthogonal experiment； fingerprint； multi-index； extraction process
doi：10.4268/cjcmm20130609
[责任编辑 曹阳阳], http://www.100md.com(张星海 李嬛 秦昆明 刘晓 汪斌 蔡宝昌)