纳米颗粒标记可视化试纸条在农药残留筛查中的研究进展(1)
1*
[摘要]农药残留严重影响中药的质量安全，国内外对于农药残留情况也愈加关注。农残快速检测技术可以用于现场快速筛查，发展迅速，尤其是纳米颗粒标记可视化试纸条作为近年来发展迅速的快速检测方法之一，备受关注。该文系统综述了用于小分子农残检测的试纸条分类和研制的关键影响因素，并通过单残留检测、多农残筛查、定量仪分析和新型纳米试纸条在农残检测的应用实例探讨了农残试纸条的研究进展，并对可视化试纸条在中药农残检测中的应用进行了展望，旨在为中药产业农残快速检测技术的发展提供参考。
[关键词]农药残留；纳米颗粒；快速检测；试纸条；中药
Advances on nanoparticlestagged visual test strips for the
rapid detection of pesticides
, 百拇医药
LIU Fuxin1，2， DOU Xiaowen1， YANG Zhixin2， LI Qian1， LUO Jiaoyang1， FAN Zhuowen2*， YANG Meihua1*
(1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine， Ministry of Education，The Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences & Peking Union
Medical College， Beijing 100193， China；
2 College of Pharmacy， Heilongjiang University of Chinese Medicine， Harbin 150040， China)
, 百拇医药
[Abstract]The residue of the pesticides affects seriously the quality and safety of traditional Chinese medicine Pesticide residue has caused evergrowing attention of people at home and abroad Rapid detection techniques used for rapid screening of pesticide residues have expanded in a fast progress As one of the fast development methods of rapid detection， visualization test strip based on nanoparticle has received much concern in recent years This article focused on the classification of detection test strips and key factors on the fabrication of nanoparticlebased visualization test strips used in small molecule pesticides Moreover， a wide application of nanoparticlestagged test strips on pesticide residue was reviewed including single residue detection， multi residue detection， as well as quantitative analysis Finally， the future application of visual test strip for detecting of pesticide residues in traditional Chinese medicine was forecasted， intending to provide the reference for rapid detection techniques on pesticide residues screening in herbal medicine industry.
, http://www.100md.com
[Key words]pesticide residue； nanoparticle； rapid detection； test strip； traditional Chinese medicine
近年來，随着农药使用量的逐渐增多，中药材中农药污染问题越来越受到国内外广泛的关注[1]。中药材中的农药残留以有机磷、有机氯、拟除虫菊酯类为主[23]，其检测方法以气相、气质联用、高效液相以及液质联用方法为主，也有使用薄层色谱法和原子吸收分光光度法进行检测[4]。然而，常规的检测技术往往需要复杂的前处理，昂贵的实验仪器，且检测时间长，显然已经不能满足当今现场快速检测的需求[5]。因此，对准确、快速且价格低廉的农残检测技术和设备的需求愈加迫切。目前，研究者们开始将目光聚焦在纳米传感技术[67]、免疫分析技术[8]等快速检测技术上。可视化试纸条是基于专属识别建立的一项具有高特异性、高灵敏度、价格低廉的适用于现场检测的快速分析技术。本文系统综述了农残检测试纸条的分类、胶体金侧流免疫层析试纸研制的关键因素、纳米颗粒标记免疫试纸条在农残检测中的应用，并展望了该快检技术在中药中农残检测的应用前景和面临的挑战，旨在为中药产业农残快速检测技术的发展提供参考。, 百拇医药(刘复新 豆小文 杨志欣李倩骆骄阳范卓文杨美华)
[摘要]农药残留严重影响中药的质量安全，国内外对于农药残留情况也愈加关注。农残快速检测技术可以用于现场快速筛查，发展迅速，尤其是纳米颗粒标记可视化试纸条作为近年来发展迅速的快速检测方法之一，备受关注。该文系统综述了用于小分子农残检测的试纸条分类和研制的关键影响因素，并通过单残留检测、多农残筛查、定量仪分析和新型纳米试纸条在农残检测的应用实例探讨了农残试纸条的研究进展，并对可视化试纸条在中药农残检测中的应用进行了展望，旨在为中药产业农残快速检测技术的发展提供参考。
[关键词]农药残留；纳米颗粒；快速检测；试纸条；中药
Advances on nanoparticlestagged visual test strips for the
rapid detection of pesticides
, 百拇医药
LIU Fuxin1，2， DOU Xiaowen1， YANG Zhixin2， LI Qian1， LUO Jiaoyang1， FAN Zhuowen2*， YANG Meihua1*
(1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine， Ministry of Education，The Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences & Peking Union
Medical College， Beijing 100193， China；
2 College of Pharmacy， Heilongjiang University of Chinese Medicine， Harbin 150040， China)
, 百拇医药
[Abstract]The residue of the pesticides affects seriously the quality and safety of traditional Chinese medicine Pesticide residue has caused evergrowing attention of people at home and abroad Rapid detection techniques used for rapid screening of pesticide residues have expanded in a fast progress As one of the fast development methods of rapid detection， visualization test strip based on nanoparticle has received much concern in recent years This article focused on the classification of detection test strips and key factors on the fabrication of nanoparticlebased visualization test strips used in small molecule pesticides Moreover， a wide application of nanoparticlestagged test strips on pesticide residue was reviewed including single residue detection， multi residue detection， as well as quantitative analysis Finally， the future application of visual test strip for detecting of pesticide residues in traditional Chinese medicine was forecasted， intending to provide the reference for rapid detection techniques on pesticide residues screening in herbal medicine industry.
, http://www.100md.com
[Key words]pesticide residue； nanoparticle； rapid detection； test strip； traditional Chinese medicine
近年來，随着农药使用量的逐渐增多，中药材中农药污染问题越来越受到国内外广泛的关注[1]。中药材中的农药残留以有机磷、有机氯、拟除虫菊酯类为主[23]，其检测方法以气相、气质联用、高效液相以及液质联用方法为主，也有使用薄层色谱法和原子吸收分光光度法进行检测[4]。然而，常规的检测技术往往需要复杂的前处理，昂贵的实验仪器，且检测时间长，显然已经不能满足当今现场快速检测的需求[5]。因此，对准确、快速且价格低廉的农残检测技术和设备的需求愈加迫切。目前，研究者们开始将目光聚焦在纳米传感技术[67]、免疫分析技术[8]等快速检测技术上。可视化试纸条是基于专属识别建立的一项具有高特异性、高灵敏度、价格低廉的适用于现场检测的快速分析技术。本文系统综述了农残检测试纸条的分类、胶体金侧流免疫层析试纸研制的关键因素、纳米颗粒标记免疫试纸条在农残检测中的应用，并展望了该快检技术在中药中农残检测的应用前景和面临的挑战，旨在为中药产业农残快速检测技术的发展提供参考。, 百拇医药(刘复新 豆小文 杨志欣李倩骆骄阳范卓文杨美华)