基于代谢组学方法的姜黄素抗抑郁作用生物标志物初步筛查(1)
[摘要]該文运用代谢组学的技术手段寻找与姜黄素干预抑郁模型大鼠相关的潜在标志物，从而探究姜黄素用于抗抑郁的作用机制。选取健康雄性SD大鼠随机分成4组，采用慢性不可预知温和应激(CUMS)刺激造模，造模2周后灌胃姜黄素(200 mg·kg-1)、文拉法辛(40 mg·kg-1)、空白组和模型组同时灌等体积的1% CMCNa生理盐水，每天1次，连续2周。采集大鼠血清并采用LC/MSITTOF方法对各组血清代谢差异进行表征，利用多元统计分析方法筛选出可能的潜在生物标志物并分析其可能的代谢通路。在分别给予姜黄素和文拉法辛后，CUMS模型组大鼠的抑郁各项指标均有显著性改善(P<005)，但姜黄素和文拉法辛组之间则无明显差异；通过PCA和PLSDA分析，经姜黄素或文拉法辛干预后，CUMS模型组大鼠体内的小分子代谢物水平体现出恢复正常的趋势，而且姜黄素组尤为明显；通过代谢组学技术手段，从中筛选得到11个与姜黄素抗抑郁功效相关的生物标志物，同时涉及到7条代谢通路。研究表明姜黄素具有确切的抗抑郁作用，其在宏观和微观层面均有体现，可与阳性药物文拉法辛相比；姜黄素在体内可能是通过影响甘油磷脂代谢、亚油酸代谢、戊糖和葡萄糖醛酸酯互变及醚脂类代谢等发挥抗抑郁功效，今后还需进一步探索。
, 百拇医药
[关键词]姜黄素； 抗抑郁； 代谢组学； 生物标志物； CUMS模型
Preliminary screening of biomarkers for curcumin′s antidepressant
effect based on metabonomics method
MA Zhijie1，2， ZHANG Wei1， DONG Jieming2， YU Xiaohong2， ZHAO Xiaomei2， PU Shibiao1*
(1 College of Pharmaceutical Science， Yunnan University of Traditional Chinese Medicine， Kunming 650500， China；
, 百拇医药
2. Beijing Friendship Hospital， Capital Medical， Beijing 100050， China)
[Abstract]To screen potential biomarkers of curcumin related to treating depression rats by using metabolomics means， so as to explore the antidepressant action mechanism of curcumin The healthy male SD rats were randomly divided into four groups Chronic unpredictable mild stress (CUMS) stimulation was conducted for modeling for 2 weeks， and then curcumin (200 mg·kg-1) or venlafaxine (40 mg·kg-1) was given by gavage administration The blank group and model group rats were given with the same volume of 1% CMCNa normal saline， once per day for two weeks The rats serum for each group was collected and LC/MSITTOF method was used to characterize the metabolic differences Also multivariate statistical analysis was used to screen possible potential biomarkers and analyze the possible metabolic pathways After administration of curcumin and venlafaxine respectively， the depression indexes of CUMS model rats were all improved significantly (P<005)， but there were no significant differences between curcumin and venlafaxine groups In PCA and PLSDA analysis after curcumin or venlafaxine intervention on CUMS model group rats， the small molecule metabolites level reflects a normal trend， and particularly for the curcumin group Through metabonomics technology， 11 biomarkers associated with curcumin antidepressant effect were screened， and at the same time seven metabolic pathways were involved The results showed that curcumin had antidepressant effects， which was evident in both macro and micro levels， comparable with positive drug of venlafaxine The antidepressant effect of curcumin may be associated with the glycerol phospholipid metabolism， linoleic acid metabolism， pentose and glucuronic acid ester and ether lipid metabolism， but still need further exploration in the future., http://www.100md.com(马致洁张祎董捷鸣于小红赵小梅浦仕彪)
, 百拇医药
[关键词]姜黄素； 抗抑郁； 代谢组学； 生物标志物； CUMS模型
Preliminary screening of biomarkers for curcumin′s antidepressant
effect based on metabonomics method
MA Zhijie1，2， ZHANG Wei1， DONG Jieming2， YU Xiaohong2， ZHAO Xiaomei2， PU Shibiao1*
(1 College of Pharmaceutical Science， Yunnan University of Traditional Chinese Medicine， Kunming 650500， China；
, 百拇医药
2. Beijing Friendship Hospital， Capital Medical， Beijing 100050， China)
[Abstract]To screen potential biomarkers of curcumin related to treating depression rats by using metabolomics means， so as to explore the antidepressant action mechanism of curcumin The healthy male SD rats were randomly divided into four groups Chronic unpredictable mild stress (CUMS) stimulation was conducted for modeling for 2 weeks， and then curcumin (200 mg·kg-1) or venlafaxine (40 mg·kg-1) was given by gavage administration The blank group and model group rats were given with the same volume of 1% CMCNa normal saline， once per day for two weeks The rats serum for each group was collected and LC/MSITTOF method was used to characterize the metabolic differences Also multivariate statistical analysis was used to screen possible potential biomarkers and analyze the possible metabolic pathways After administration of curcumin and venlafaxine respectively， the depression indexes of CUMS model rats were all improved significantly (P<005)， but there were no significant differences between curcumin and venlafaxine groups In PCA and PLSDA analysis after curcumin or venlafaxine intervention on CUMS model group rats， the small molecule metabolites level reflects a normal trend， and particularly for the curcumin group Through metabonomics technology， 11 biomarkers associated with curcumin antidepressant effect were screened， and at the same time seven metabolic pathways were involved The results showed that curcumin had antidepressant effects， which was evident in both macro and micro levels， comparable with positive drug of venlafaxine The antidepressant effect of curcumin may be associated with the glycerol phospholipid metabolism， linoleic acid metabolism， pentose and glucuronic acid ester and ether lipid metabolism， but still need further exploration in the future., http://www.100md.com(马致洁张祎董捷鸣于小红赵小梅浦仕彪)