盐酸二甲双胍对成骨细胞增殖、BMP-2及Cbfa-1基因表达的影响(1)
 |
| 第1页 |
参见附件(1975KB，3页)。
[摘要] 目的 初步研究二甲双胍(MF)在体外对小鼠颅盖骨成骨细胞增殖、骨形态发生蛋白-2(BMP-2)及核心结合因子(Cbfa-1)mRNA表达的影响，探讨二甲双胍对骨代谢的可能作用机制。 方法 (1)分离培养原代颅盖骨成骨细胞并对其进行鉴定。(2)以乳鼠成骨细胞为体外实验模型，不同浓度(0、50、100、200、400 μmol/L)的MF干预体外培养的成骨细胞24 h后，MTT法检测成骨细胞的增殖能力，实时荧光定量PCR法检测成骨细胞BMP-2及Cbfa-1基因表达。 结果 二甲双胍干预成骨细胞24 h后，可促进成骨细胞的增殖，在浓度400 μmol/L的OD值最大为0.298±0.047(P < 0.05)；可促进 BMP-2及Cbfa-1 mRNA的表达，呈剂量效应关系。 结论 二甲双胍可促进成骨细胞的增殖和分化，可能通过调节BMP-2及Cbfa-1的表达，从而促进骨的形成。
[关键词] 二甲双胍；成骨细胞；骨形态蛋白-2；核心结合因子-1
[中图分类号] R361+.3 [文献标识码] A [文章编号] 1674-4721(2012)03(a)-0021-03
Effect of metformin (MF) on the proliferation, bone morphogenetic protein-2 (BMP-2) and core binding factor al (Cbfa-1) gene expression in cultured rat osteoblast
LI Yinyu DONG Jin
Department of Endocrinolong, the First Hospital of Shanxi Medical University, Taiyuan 030001, China
[Abstract] Objective To study the effect of metformin on proliferation, bone morphogenetic protein-2 (BMP-2) and core binding factor-1 (cbfa-1) gene expression in cultured rat calvarial osteoblasts. And the possible mechanism of action on osteoblats was investigated. Methods (1) Primary calvarial osteoblasts were isolated, cultured and identified. (2) The rat osteoblasts were cultured in vitro, cultured osteoblasts were treated with metformin in different concentrations (0, 50, 100, 200, 400 μmol/L) after 24 hours. Cell proliferation was assessed by MTT and the expression of BMP-2 and Cbfa-1 mRNA were analyzed by real-timuantitative PCR. Results Osteoblasts were treated with metformin after 24 hours, Metformin could promote osteoblast proliferation, the OD of 400 μmol/L concentrations was the biggest, which was 0.298±0.047 (P < 0.05), and the expression of BMP-2 and Cbfa-1 mRNA were increased, in concentration-dependent manner. Conclusion Metformin induces the proliferation, differentiation of osteoblast probably by increasing the expression of BMP-2 and Cbfa-1 mRNA.
[Key words] Metformin; Osteoblast; Bone morphogenetic protein-2 (BMP-2); Core binding factor-1 (cbfa-1)
近来降糖药物对骨骼的影响日益受到人们的关注。二甲双胍作为一种经典的口服降糖药，已被广泛应用于2型糖尿病及其他代谢性疾病的治疗。新近的研究发现二甲双胍能够降低1型和2型糖尿病患者骨折的发生率，表明其对骨骼能够产生保护作用[1]。但具体的作用机制尚不明确。本研究通过观察二甲双胍对体外培养的原代颅盖骨成骨细胞增殖、BMP-2及Cbfa-1基因表达的影响 ......
您现在查看是摘要介绍页，详见PDF附件(1975KB，3页)。